The best Side of cytometer
The best Side of cytometer
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Info-enriched characterization of cell populations. Morphological data from photographs can add on the richness of flow cytometry data. By way of example, the determine demonstrates an if not conventional apoptosis assay working with Annexin V and PI, introducing cell imaging to characterize cells in each population to reveal morphologically unique options. These insights couldn't are attained from multiplex staining alone.
As samples are obtained within the Attune CytPix Flow Cytometer, the significant-pace brightfield digicam captures and merchants pictures of detected situations in a fee approximately six,000 pictures for every 2nd, depending upon the flow rate and graphic dimension. For bigger flexibility, Attune Cytometric Software program helps you to change the picture capture frequency as necessary and choose a particular gate for image seize, if expected.
(B) Uncooked 264.seven macrophages are frequently annotated inaccurately with regard to particle rely due to their complexity and noncircular borders. The quantity of singlets available for Examination raises after the consumer trains and employs a more precise product.
The award-profitable Invitrogen Attune CytPix Flow Cytometer brings together acoustic focusing with a significant-speed brightfield camera for simultaneous significant throughput flow cytometry and higher resolution brightfield imaging.
"An image just isn't rather worthy of one thousand dots, but it seriously really helps to see illustrations or photos. It is excellent to find out visuals that correlate with staining. By way of example, AnnexinV-favourable cells which might be morphologically unique than balanced cells, or CD14+ monocytes which might be more substantial and much more textured than CD3+/CD4+/CD14- lymphocytes.
On top of that, it really is illuminating to check out the quantity of doublets slip by way of regular singlet gates, and terrific to confirm the position of unpredicted double-optimistic gatherings in my analysis."
Pair your Attune flow cytometer with an autosampler for elevated workflow effectiveness, and increase robotic integration for max throughput.
Our experts will design and style panels to suit your needs as many times you may need—free of charge, no product purchase wanted.
The Attune NxT cytometer is the responsible workhorse instrument your lab can depend on for consistent and dependable efficiency. The Attune CytPix design features a significant-speed brightfield digicam and AI-pushed image Examination computer software, with modern upgrades including device Studying resources and an intuitive person interface, enabling Improved data precision, accuracy, and faster insights into sample biology.
Probably most importantly, these events have cells of clearly unique phenotypes which may lead to incorrect conclusions regarding double optimistic occasions (specifically in rare populations).
AI-pushed automated impression analysis Together with the Attune Cytometric Software package interprets event characteristics into unique morphology parameters that could be combined with regular fluorescence and scatter parameters to expose extremely reproducible populace adjustments and special subsets.
We Beforehand shown the strength of imaging CAR-T/Ramos mobile interactions. Permit’s examine just the populace of biggest interest, the double positive get more info gatherings, to learn more. We will now use prolonged morphological parameters (below, circularity and skewness of intensity) to further examine the attributes of such populations and refine gating on these situations, expanding details robustness.
Leverage the power of Highly developed technological innovation and innovation to speed up your discoveries. Have confidence in from the Attune Flow Cytometer relatives to deliver the trustworthiness, velocity, and precision that established your work apart.
No wash/no lyse leukocyte Assessment with imaging. Cells had been acquired from 24-hour-aged blood by dilution in 1 mM EDTA ( Qualified UX/UI permits buyers to teach AI styles for expanded applications. (A) The “Practice Design” element delivers annotation tools and genuine-time model training accuracy.
At the guts of every Attune Flow Cytometer lies our revolutionary acoustic concentrating engineering, which utilizes audio waves to meticulously align cells in the flow cell. This modern technique surpasses traditional hydrodynamic strategies, resulting in more rapidly processing speeds, Extraordinary knowledge quality, and elevated sample throughput.
As the cell line experienced Formerly been analyzed on the Attune CytPix Flow Cytometer, the researchers went back again to the photographs and had been capable to doc the microbial infection no less than 5 times earlier.
Agilent Value Assure guarantees you at the least a decade of instrument use from your date you purchase. If not, We'll credit you Together with the residual value of the method towards an upgraded product.
Here, we present that by gating procedures working with picture-centered quantitative parameters can distinguish interacting cells from coincident activities to more precisely evaluate interacting cells.